nr2b  (Alomone Labs)

Journal: PLOS One

Article Title: The role of DAAO in cognitive impairment of offspring mice induced by arsenic exposure during early developmental stage

doi: 10.1371/journal.pone.0333414

Figure Lengend Snippet: (A, C, E) Real-time RT-PCR was applied to detect NR1, NR2A and NR2B mRNA levels. (B, D, F) Protein levels of NR1, NR2A and NR2B were measured by WB. Images were the representative blots and statistical analysis of protein levels. Data were shown as mean ± SD, n = 6. P < 0.05, * vs. the control, # vs. NaAsO 2 group.

Article Snippet: Antibodies were DAAO (1:500), NR1 (1:500) (Boster, China), SR (1:500), NR2A (1:500), NR2B (1:500) (Proteintech, China), SYP (1:1000), PSD95 (1:1000) (Abcam, UK), β-actin (1:10000) (Santa Cruz, USA).

Techniques: Quantitative RT-PCR, Control

Journal: Translational Psychiatry

Article Title: Chronic postoperative pain induces contextual fear extinction dysfunction through hippocampal NMDAR/BDNF/TrkB signaling pathway in mice

doi: 10.1038/s41398-025-03417-0

Figure Lengend Snippet: A Representative Western blot analysis of NR2B、NR2A、P-ERK、ERK、P-CREB and CREB in hippocampus. β-Tublin was included as loading control. B Quantitative analysis of NR2B levels (n = 4). C Quantitative analysis of NR2A levels (n = 4). D Quantitative analysis of P-Erk1/2 levels (n = 4). E Quantitative analysis of P-CREB levels (n = 4). F Representative Western blot analysis of PSD-95 in hippocampus. β-Tublin was included as loading Control. G Quantitative analysis of PSD-95 levels (n = 4). H Experimental flow chart. I Representative map of dendritic spine density of neurons in hippocampus. J Quantitative statistics of dendritic spine density (n = 6). Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01.

Article Snippet: The membrane was blocked using TBST containing 0.1% Tween-20 and 5% skim milk, followed by incubation with the following antibodies: anti-NR2A(1:1000, #4205S, Cell Signaling Technology, USA); anti-NR2B(1:1000, #14544S, Cell Signaling Technology, USA); anti-ERK1/2(1:1000, #4695 T, Cell Signaling Technology, USA); anti-P-p44/42MAPK(1:1000, #9101S, Cell Signaling Technology, USA); anti-CREB(1:1000, #9197 T, Cell Signaling Technology, USA); anti-P-CREB(1:1000, #9198S, Cell Signaling Technology, USA); anti-BDNF(1:1000, #EPR1292, Abcam, USA); anti-β3-Tubllin(1:1000, #5666S, Cell Signaling Technology, USA); anti-PSD-95(1:1000, #2507S, Cell Signaling Technology, USA); anti-TrkB(1:1000, #4603, Cell Signaling Technology, USA); anti-TrkB(B-3) (1:500, #sc-7268, Santa Cruz Biotechnology, USA).

Techniques: Western Blot, Control